High-performance Multi-band LED-Fluorescence Microscope with Cooled 6MP CCD Imaging System

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$5,094.00

High-performance Multi-band LED-Fluorescence Microscope with Cooled 6MP CCD Imaging System

Description

Overview

The T800-FLLED upright fluorescence microscope uses a cutting-edge 3-LED illuminator for variable-band excitation. Oufitted with the widely-used green (G1), blue (B1), and ultraviolet (UV2) excitation-bands, it can be used for a wide variety of common labeling or autofluorescence. Unlike single-LED fluorescence illuminators, the three LEDs are cycled using a multi-filter turret, eliminating the need to exchange light-sources. Each LED provides a precise excitation-source to eliminate non-relevant light, as well as providing maximum intensity at the targeted wavelength. Another major benefit of LED illumination is the elimination of long warm-up times and other care required by fluorescent light-sources such as mercury-vapor.

The infinity-corrected optical system includes upgraded plan-fluor objectives, which provide edge-to-edge sharpness with higher resolution, improved color-correction, and improved short and long wavelength transmission. The reversed quintuple-nosepiece is equipped with 4X, 10X, 20X, 40X, and 100X plan semi-apochromatic lenses. The 10X plan, high-eyepoint eyepieces provide a compounded magnification range of 40X to 1000X. The Koehler illumination-system is designed for precision, with a variable-NA swing-condenser allowing the use of high and low magnifications with full, even coverage. The robust frame offers excellent stability and ergonomics, with coaxial coarse and fine focusing, as well as coaxial stage-translation knobs providing fluid control.

The ultra-sensitive 6MP digital camera provides a temperature-controlled, low-light imaging solution, ideal for fluorescence microscopy. The large, highly-sensitive CCD sensor produces low-noise images, thanks in-part to a controllable internal cooling unit. The camera captures still images and video, and allows you to view a live stream on your PC. Because of the CCD’s “global shutter” design, it eliminates rolling-shutter artifacts. With software available for Windows, Mac, and Linux, it’s a multi-platform imaging solution. The included Windows software offers image-development and measurement tools, as well as advanced compositing features such as image-stitching and extended-depth-of-focus. Independent preview and capture resolutions allow you to view high-speed video while capturing high-definition images. For Mac and Linux, there is a lite version with limited processing features.

Fluorescence

Filters
Filter Set Chart Excitation Fiter Dichromatic Mirror Emission Fiter Common Stains
UV2 340-390nm 400nm 420nm Alexa Fluor (350, 405), BFP, DAPI, Dopamine, Hoechst (33258, 33342, 34580)
B1 450-490nm 495nm 500-550nm Acridine Orange (DNA/RNA), Alexa Fluor (488, 500, 514, 610, 647), Chlorophyll, Cy2, DiO, GFP, Rhodamine (110, 123)
G1 540-580nm 585nm 590-665nm Texas Red®, Texas Red® X, Cy3.5, Mito Tracker® Red
LED Excitation
Band Peak Wavelength
UV2 385nm
B1 470nm
G1 560nm

Specifications

Optical System Infinity-corrected
Head Trinocular, 30 ° incline, 360 ° rotatable
Interpupillary Adjustment Compensation-free gemel, 48-75mm
Eyepieces Plan 10X, high-eyepoint, FN 22mm, 30mm mount
Photo-port 1X focus-adjustable C-mount
Nosepiece Quintuple reversed
Objective Lenses 4X, 10X, 20X, 40X (spring), 100X (spring, oil)
Focusing System Coaxial coarse and fine focus, upper limit-stop, tension-control
Focus Travel 30mm, 0.002mm fine-adjustment
Stage Double-layer mechanical 185mm x 165mm
X-Y Translation Range 80mm x 55mm
Sub-stage Condenser-holder Rack-and-pinion, centerable
Condenser Achromatic swing condenser, NA1.2/0.22
Transmitted Illumination 3W LED, 6V2A, continous adjustment
Fluorescence Excitation Illumination 3 x 3W LED: UV2(385nm), B1(470nm), G1(560nm)

Objective Lenses

Magnification Corrections NA Working Distance Cover-glass Thickness
4X Plan semi-apochromatic 0.13 15.13mm 0.17mm
10X Plan semi-apochromatic 0.30 8.53mm 0.17mm
20X Plan semi-apochromatic 0.50 2.33mm 0.17mm
40X Plan semi-apochromatic 0.75 0.55mm 0.17mm
100X Plan semi-apochromatic 1.28 0.21mm 0.17mm

Camera

Camera Sensor Type Sensor Size Pixel Size Resolution Framerates Sensitivity Connectivity
MF603C-CCD HAD CCD 1″ Class14.6×12.8mm 4.54µm 6.0MP 14fps @ 2748×10927.5fps @ 2748×2200 880mV (1/30s) USB 3.0

Components

Objectives
Plan Achromatic Objectives ○
Plan Semi-apochromatic Objectives •
Plan Apochromatic Objectives ○
Eyepieces
10X FN22 •
15X ○
20X ○
25X ○
Condensers
Brightfield Swing Condenser (NA1.2/0.22) •
Dry Darkfield Condenser (NA0.90) ○
Oil Darkfield Condenser (NA1.25) ○
Phase-contrast Turret ○
Heads
Binocular Head ○
Trinocular Head •
Tilting Erect-image Trinocular Head ○
Photo-ports(Trinocular Heads Only)
1X C-mount Adjustable Photo-port •
0.5X C-mount Adjustable Photo-port ○
0.3X C-mount Adjustable Photo-port ○
23mm Adjustable Photo-port ○
• included ○ optional – not available

Additional information

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High-performance Multi-band LED-Fluorescence Microscope with Cooled 6MP CCD Imaging System

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